Next Article in Journal
Mass Spectrometric Analysis of Antibody—Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization
Previous Article in Journal
Synthesis of Block Copolymer Brush by RAFT and Click Chemistry and Its Self-Assembly as a Thin Film
Previous Article in Special Issue
Mass Spectrometry and Structural Biology Techniques in the Studies on the Coronavirus-Receptor Interaction
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Section
Special Issue
Volume
Issue
Number
Page
 
Logical OperatorOperator
Search Text
Search Type
 
add_circle_outline
remove_circle_outline
 
Open AccessReview

Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

by , * and
Divisions of Laboratory Medicine, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo 173-8610, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Pierluigi Luigi Mauri, Martina Marchetti-Deschmann and Diana Canetti
Molecules 2020, 25(20), 4775;
Received: 11 September 2020 / Revised: 15 October 2020 / Accepted: 15 October 2020 / Published: 17 October 2020
(This article belongs to the Special Issue Mass Spectrometric Proteomics II)
Mass spectrometry (MS), a core technology for proteomics and metabolomics, is currently being developed for clinical applications. The identification of microorganisms in clinical samples using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is a representative MS-based proteomics application that is relevant to daily clinical practice. This technology has the advantages of convenience, speed, and accuracy when compared with conventional biochemical methods. MALDI-TOF MS can shorten the time used for microbial identification by about 1 day in routine workflows. Sample preparation from microbial colonies has been improved, increasing the accuracy and speed of identification. MALDI-TOF MS is also used for testing blood, cerebrospinal fluid, and urine, because it can directly identify the microorganisms in these liquid samples without prior culture or subculture. Thus, MALDI-TOF MS has the potential to improve patient prognosis and decrease the length of hospitalization and is therefore currently considered an essential tool in clinical microbiology. Furthermore, MALDI-TOF MS is currently being combined with other technologies, such as flow cytometry, to expand the scope of clinical applications. View Full-Text
Keywords: bacterial identification; blood culture; clinical proteomics; matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS); sample preparation bacterial identification; blood culture; clinical proteomics; matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS); sample preparation
Show Figures

Figure 1

MDPI and ACS Style

Tsuchida, S.; Umemura, H.; Nakayama, T. Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology. Molecules 2020, 25, 4775.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop